4',5'-二溴熒光素
物理性質
物性數據:
1. 熔點(℃):270-273
貯存方法
貯存:
將密器密封,儲存密封的主藏器內,并放在陰涼, 干爽的位置。
分子結構數據
五、分子性質數據:
1、 摩爾折射率:103.43
2、 摩爾體積(m3/mol):232.9
3、 等張比容(90.2K):741.7
4、 表面張力(dyne/cm):102.8
5、 極化率(10
計算化學數據
四、計算化學數據:
1、 疏水參數計算參考值(XlogP):4.8
2、 氫鍵供體數量:2
3、 氫鍵受體數量:5
4、 可旋轉化學鍵數量:0
5、 拓撲分子極性表面積(TPSA):76
6、 重原子數量:27
7、 表面電荷:0
8、 復雜度:585
9、 同位素原子數量:0
10、 確定原子立構中心數量:0
11、 不確定原子立構中心數量:0
12、 確定化學鍵立構中心數量:0
13、 不確定化學鍵立構中心數量:0
14、 共價鍵單元數量:1
安全信息
危險運輸編碼: UN 2811 6.1/PG 3
危險品標志: 有毒
安全標識:S45
危險標識:R25
作用與用途
Analysis Note The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining.
性質與穩定性
1.脫氧核糖核酸,脂蛋白和免疫電泳作用。生化研究、免疫擴散等的支持物。生物學、免疫學、生物化學和微生物學研究。臨床醫學上用于乙型肝炎抗原測定。血脂電泳分析。甲胎球蛋白測定。肝炎、肝癌和心血管等疾病的診斷。
2.凝膠過濾法分離病毒、噬菌體、核糖核酸、脫氧核糖核酸、高分子蛋白質及多糖。
3.用于電泳支持物。生化試劑一般用于乙肝抗原、甲胎球蛋白的測定。