微球菌核酸酶
作用與用途
Biochem/physiol Actions Hydrolyzes 5′-phosphodiester bonds of DNA.
Other Notes Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.
Unit Definition One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.
貯存方法
-20°C密閉，避光，通風干燥處
