物理性質

性狀：淺紅色或淺黃色凍干粉。
溶解性：溶于水，最適pH值7.0-7.5(豬腎、淀粉酶)或7-8(米曲酶)。

作用與用途

Analysis Note Protein determined by biuret.
Unit Definition One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.
Biochem/physiol Actions The enzyme hydrolyzes a variety of N-acyl-L-amino acids and dehydropeptides containing dehydroalanyl residues. N-acyl-D-amino acids and dehydropeptides other than dehydroalanine are not hydrolyzed. The following compounds are hydrolyzed by acylase I: chloroacetyl-L-amino acids, L-leucinamide, chloroacetyldehydroalanine, glycyl-L-amino acids, and glycyldehydroalanine. Aminoacylase is widely used as a reagent to resolve amino acid racemates. The Km for acyl amino acids have been reported in the literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), and Ac-L-Phe (5.5 mM).
Aminoacylase from pig kidney is activated by Zn2+ and Co2+ and inhibited by SH blocking reagents, disulfide reducing reagents, N-tosyl-L-alanine,
DL-norleucine, and N-tosyl-L-lysine chloromethylketone.

性質與穩定性

用以分離D-型和L-型氨基酸。

合成方法

以豬腎、淀粉酶或米曲霉為原料，經勻漿、離心去沉淀后，用硫酸銨分級沉淀，經水透析、離心后得粗酶液，再分別用丙酮、乙醇分級沉淀、DEAE-纖維素柱層析純化后得產品。

貯存方法

密封-20℃干燥保存。