物理性質

性狀：凍干粉末。
溶解性：溶于水

作用與用途

Application Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Biochem/physiol Actions In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Unit Definition One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
Physical form Lyophilized powder containing sucrose and Tris buffer salts

性質與穩定性

二、用途
用于生化研究。

貯存方法

一、貯存
應充氬氣密封于0℃以下（粉末）和4℃（懸浮液）保存。

計算化學數據

計算化學數據：
1、 疏水參數計算參考值（XlogP）：6.1
2、 氫鍵供體數量：3
3、 氫鍵受體數量：4
4、 可旋轉化學鍵數量：12
5、 拓撲分子極性表面積（TPSA）：73.1
6、 重原子數量：38
7、 表面電荷：0
8、 復雜度：739
9、 同位素原子數量：0
10、 確定原子立構中心數量：0
11、 不確定原子立構中心數量：0
12、 確定化學鍵立構中心數量：0
13、 不確定化學鍵立構中心數量：0
14、 共價鍵單元數量：1